Research Fellow in Metals Analysis and Bioimaging

We are looking to recruit an experienced and motivated Research Fellow to play a central role in developing and maintaining the new “London Metallomics Facility”. This facility is funded by the Wellcome Trust and this role will be crucial in providing scientific support and also networking and promoting the centre. Click here for more details.

PhD studentship

PhD studentship “Identifying regulators of tissue regeneration by in vivo imaging in the zebrafish”

 

Supervisory team: Dr. Robert Knight (robert.knight@kcl.ac.uk), Prof. Christer Hogstrand (christer.hogstrand@kcl.ac.uk)

 

Sponsor: NC3Rs

 

Eligibility Home/EU (residency rules apply)

 

Funding available tuition fees £4121 per year, stipend of £16,296 per year, duration: 3 years.

 

Abstract

This proposal aims to use a zebrafish model of muscle regeneration in order to identify the signals between muscle stem cells (muSCs) and inflammatory cells. The purpose is to determine which signals regulate their interactions during regeneration and so control muscle repair. Specific Aims to be tested include:

  1. determine how key pro- and anti-regenerative signals regulate muSCs and mø responses to tissue injury
  2. identify candidate signalling pathways controlling muSCs and mø interactions during muscle regeneration
  3. functionally test the role of candidate cell signalling pathways in controlling muSC responses to injury

To address these aims we will use transgenic zebrafish for gene over-expression and visualisation of cells during regeneration. Imaging of stem cell and macrophage responses to injury will be performed by confocal and multiphoton microscopy. To identify complementary signals and receptors expressed between muSCs and inflammatory cells, gene expression profiling will be performed on cells isolated by flow cytometry using RNA sequencing. Candidate secreted proteins and their respective receptors that could act to signal between muSCs and inflammatory cells will be identified using computational analyses of sequencing data. These candidate molecules will then be tested in vivo by over-expression under the control of tissue specific promoters and their affects measured by live cell imaging.

 

Deadline for applications: 31st Jan 2017

Expected start date: 1 October 2017

 

Post-doctoral research fellow, the LNS Phytase Study, University of California, Davis, based in Keneba, The Gambia

We are implementing a randomized, controlled trial to determine the efficacy of adding exogenous phytase to small-quantity lipid-based nutrient supplements (SQ-LNS) on the absorption of zinc among young children in The Gambia. We are seeking to hire a scientific field coordinator to assist with the rigorous implementation of this RCT, which employs a triple zinc stable-isotope ratio tracer technique to assess zinc absorption from meals containing SQ-LNS, and data collection concerning baseline anthropometric and nutritional status. The ideal candidate would be a post-doctoral scientist with background in Nutrition, International Nutrition, or Medicine with interest in one or more of the foregoing topical areas and prior field experience in a lower income country.

Nature of position

This position involves study planning, staff training and supervision (approximately 5 individuals) and quality assurance of data collection procedures. Possibilities are available for data analysis and interpretation, and related report writing. The project takes place in Keneba, The Gambia, at the Medical Research Council, The Gambia Unit/International Nutrition Group field station; and the incumbent will be expected to reside at the field site during the period of data collection (4-6 months). The approximate start date will be January 1, 2017. The post-doctoral scholar is hired through the University of California, Davis. Salary is based on experience, per NIH post-doctoral scholar stipend scales and the UC Davis policy.

Major responsibilities

The incumbent will oversee data collection for the study, as described above. The incumbent will be responsible for on-site supervision of field personnel, including oversight of meal preparation, administration of stable isotope doses, and biological samples (urine and blood) collection. The incumbent will be responsible for technical guidance of field personnel, quality control of data collection, and coordination of data management with the IT team. After completion of the data collection, the incumbent may take the lead in writing a scientific publication on study results.

This position requires a doctoral degree (PhD) in biomedical sciences, human nutrition or public health (with emphasis in epidemiology, infectious disease, or nutrition), or a degree in medicine (MD) and experience carrying out clinical or community-based studies related to micronutrient deficiencies. Advanced graduate students with prior experience in the stable isotope technique will be considered. English language fluency is an important prerequisite.

For additional information, contact Dr. Ryan Wessells, Department of Nutrition, University of California, Davis (krwessells@ucdavis.edu). To apply, please send a current CV, cover letter and contact information for references to Dr. Wessells by December 1, 2016.